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SimpleStepELISA?technologyemployscaptureantibodiesconjugatedtoanaffinitytagthatisrecognizedbythemonoclonalantibodyusedtocoatourSimpleStepELISA?plates.ThisapproachtosandwichELISAallowstheformationoftheantibody-analytesandwichcomplexinasinglestep,significantlyreducingassaytime.SeetheSimpleStepELISA?protocolsummaryintheimagesectionforfurtherdetails.OurSimpleStepELISA?technologyprovidesseveralbenefits:
-Single-washprotocolreducesassaytimeto90minutesorless-Highsensitivity,specificityandreproducibilityfromsuperiorantibodies-Fullyvalidatedinbiologicalsamples-96-wellsplatebreakableinto12x8wellsstrips
TherearethreeclassesofimmunoglobulinsinHamster:IgA,IgM,andIgG.IgGisthemostabundantimmunoglobulininserumandisequallydistributedinbloodandtissue.InHamster,theIgGclassisfurtherdividedintotwosubclasses:IgG1,IgG2;howeversomeresearchsuggeststhatathirdsubclass,IgG3,isalsofoundinoutbredstrains,andonlysomeinbredstrainsofHamsterserum.Thegeneralimmunoglobulinstructureiscomposedoffourpolypeptidechains,twoheavyandtwolightchainslinkedtogetherandtoeachotherbydisulfidebonds,creatingatetramericquaternarystructure.TheresultingtetramercreatestwoidenticalhalveswhichtogetherformaY-likestructure.Whiletheamino-terminalportionsthatexhibitshighlyvariableamino-acidcompositionareinvolvedinantigenbinding,theCterminalconstantpartsareinvolvedincomplementbinding,placentalpassageandbindingtocellmembrane.
IgGisinvolvedinresponsetoaforeignantigenandthepresenceofIgGusuallysignifiesamatureantibodyresponse.IgGhasamolecularweightofabout150kDa,canbindtomanypathogensandalsoplaysanimportantroleinantibodydependentcell-mediatedcytotoxicity.Typicallyhamsterserumandplasmasamplescontainabout7to10mg/mlofIgG.
“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
LinearityofIgGhamsterbytestinghamsterSerumassayedatadilutionof1:2Million(undiluted),andinterpolatingtheundilutedanddilutedamountsandmultiplyingbythedilutionfactors(D.F.)isplotted.
Background-subtracteddatavalues(mean+/-SD)aregraphed.
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