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ThePMM1proteiniskeytothesynthesisofGDP-mannoseandpolyethyleneglycol-phosphate-mannoseandisessentialforthemannosyltransferreaction.Itsenzymaticactivitycontributestothebiosynthesisofmannose-containingglycoconjugates,whicharecriticalforproteinglycosylationandrelatedcellularprocesses.PMM1Protein,Human(His)istherecombinanthuman-derivedPMM1protein,expressedbyE.coli,withC-6*Hislabeledtag.ThetotallengthofPMM1Protein,Human(His)is262a.a.,withmolecularweightof~49.0kDa.
Human
E.coli
C-6*His
MAVTAQAARRKERVLCLFDVDGTLTPARQKIDPEVAAFLQKLRSRVQIGVVGGSDYCKIAEQLGDGDEVIEKFDYVFAENGTVQYKHGRLLSKQTIQNHLGEELLQDLINFCLSYMALLRLPKKRGTFIEFRNGMLNISPIGRSCTLEERIEFSELDKKEKIREKFVEALKTEFAGKGLRFSRGGMISFDVFPEGWDKRYCLDSLDQDSFDTIHFFGNETSPGGNDFEIFADPRTVGHSVVSPQDTVQRCREIFFPETAHEA
Approximately49.0kDa
Greaterthan95%asdeterminedbyreducingSDS-PAGE.
<1EU/μg,determinedbyLALmethod.
Speciescross-reactivitymustbeinvestigatedindividuallyforeachproduct.Manyhumancytokineswillproduceaniceresponseinmousecelllines,andmanymouseproteinswillshowactivityonhumancells.Otherproteinsmayhavealowerspecificactivitywhenusedintheoppositespecies.
Volume(toaddtovial)=Mass(invial)÷DesiredReconstitutionConcentration
Concentration(start)×Volume(start)=Concentration(final)×Volume(final)
Thisequationiscommonlyabbreviatedas:C1V1=C2V2
SpecificActivity(Unit/mg)=106÷BiologicalActivity(ED50)
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