上海金畔生物科技有限公司代理HarlanTeklad动物饲料全线产品，欢迎访问HarlanTeklad动物饲料官网了解更多信息。
Tekladmedicatedlabanimaldietsaremanufacturedeitheratasubsidiaryplantoratourcustomresearchdietfacility.ContactcustomerserviceoryourlocalInotivaccountrepresentativeforpricingandavailabilityofstockeddiets.Contactanutritionisttodiscusscustomizedversionsoftheseorothermedicateddiets.
Medicateddietsmustbeusedasdirectedbyaveterinarian.
CustomdietswithIvermectin,Uniprim,andFenbendazoleareavailable.Modificationsinclude:differentbasediet,coloringwithfooddyes,andotherdosingconcentrationsbasedonyouruniquerequirements.Contactanutritionisttodiscussyourneeds.
Othermedicationsandcompoundssuchasdoxycyclineandtamoxifenmaybeaddedtodietsatourcustomresearchdietfacility.Visitourcustomresearchdietssiteorcontactusforadditionalinformation.
Standardnaturalingredientdietsfulfillmanyeverydayneedsoflaboratoryanimalcoloniesforbreeding,growthandmaintenance.Thesedietsconsistofrelativelyunrefinedagriculturalcommoditiessuchasgrains,grainby-products,concentratedplantproteinsources,animalproteins,andvitamins,mineralsandfats.
Dietsbyspecies:
Contactusataskanutritionist@inotivco.comtolearnmoreaboutthedifferenceofusingTekladlaboratoryanimaldiets.
Whencarefullychosen,standardnaturalingredientdietwillsupportmanyresearchobjectives.
Note:Standardnaturalingredientdietsmayalsobereferredtosimplyas“standard,”“grain-based,”or“chow.”
Naturalingredientsarechemicallycomplex,containingavarietyofnutritiveandnon-nutritivecomponents,bothofwhichcanhavesignificanteffectsonyourresearch.Weworktominimizevariationinnutrientandnon-nutrientlevelsthroughthecombinationof:
Withthishigh-qualityproductionprocess,youcanexpectmoreconsistentresults.
Note:Terminologyusedtodescribelaboratoryanimaldietsisnotconsistentacrosstheindustry.Inthiscontext,weusethephrase“naturalingredient”todenotetherelativelackofprocessingoftheingredients.Naturalingredientdietsmightalsobereferredtoas“standard”,“grain-based”or“chow”—termsthatmayconveysomeinformationaboutthenatureofthedietorhowitisusedbutmaynotprovideenoughclarityforyourpurposes.
品牌
货号
品名
chromogenix
0082072039
PeptideInstitute
3155-v
Boc-Arg-Val-Arg-Arg-MCA
zyagen
GG-150
GoatGenomicDNA
GB-110F
BovineGenomicDNA,Female
GS-190F
SheepGenomicDNA,Female
OmmScientificInc
Autoinducer-2
(S)-4,5-Dihydroxy-2,3-pentandione(DPD,AI-2)Autoinducer-2
Aldevron
5006
gWiz-GFP
5007
stemcell
09650
StemSpanSFEM
cordenpharma
LP-R4-039
MPEG-2000-DSPECAS:147867-65-0
pblassaysci
21410-1
Anti-HumanIFNBetaAntibody,CloneMMHB-2(MAb)500ug
Cygnus
F030
F650R
HEK293HCPELISAKit
polysciences2017
24885-2
PolyethylenimineHClMAX,Linear,Mw4,000(PEIMAX4000)
07307-15
PolybeadMicrospheres0.50m
trilink
L-7206
CleanCapCas9mRNA(modified)
bachem
H-7442.0500
Amyloid-Protein(1-42)(HFIP-treated)
EXBIO
A6-222-T100
AlexaFluor647
NIST
SRM2921
HumanCardiacTroponinComplex
AcudermInc
P1225
12mmAcu-PunchBoxof25
Vectorlabs
H-1500
VECTASHIELDHardSetwithDAPI
omicronbio
SUC-006
[UL-13C12]sucrose(-D-[UL-13C6]fructofuranosyl-D-[UL-13C6]glucopyranoside)
signalchem
N16-11G-10
TRKA,Active
N17-11G-10
TRKB,Active
N18-11H-10
TRKC,Active
SekisuiDiagnostics
299F
SPECTROFLUORFIXa
BECKMAN
A30341
SDS-MWGELBUFFERMULTIPACK
A47775
ELECTRODEREPLACEKITPA800EN
359976
COOLANTFORCAPELECTRO
477441
CAPILLARIES
Shino-TestCorporation
326054329
HMGB1ELISAKitII
MileniaBiotec
MGHD1
MileniaHybriDetect
MGHD21
MileniaHybriDetect2T
LifeSensors
4010
SUMOProtease1
Worthington
LS003126
Papain,Suspension
LS003119
Papain,Lyophilized
IBALifeSciences
2-4010-010
Strep-TactinXTSuperflow50%suspension
alfa
L04801
Cholesterylchloroformate
A10409
Ninhydrin,99%
CarnaBiosciences,Inc.
01-140
PKC(PRKCQ)
polymersource
P19087A-SNH2
Poly(styrene),-amino-terminated(nosilaneoramidelinkage)
LS002139
DeoxyribonucleaseI
L-1040-10
Lensculinarisagglutinin(LCA,LcH)
L-1040-25
dshb
G3G4(anti-BrdU)
5-bromo-2-deoxyuridineBrdU
bangslabs
CP01004（CP01N）LOT:12833
StreptavidinCoatedMicrospheres
FormuMax
F70101C-N-2
Clophosome,ClodronateLiposomes(Neutral)
ListBiological
181
880126C
DSPE-PEG(2000)Maleimide|880126
PepTechCorporation
AD943-2
(S)-3-Amino-3-(3-bromo-phenyl)-propionicacid
moleculardimensions
MD1-101
TheDurhampHScreen
polysciences
BLI817A-1
QuantumSimplyCellularanti-RatIgG
BLI81-1
QuantumSimplyCellularanti-MouseIgG
Jenabioscience
NU-1103S
Etheno-ATP(ε-ATP)
Biolegend
351005
APCanti-humanCD276(B7-H3)
358103
PEanti-humanB7-H4
345015
PerCP/Cy5.5anti-humanCD366(Tim-3)
Alomone
ACC-003-50ul
Anti-CaV1.2
bangslab
817
815
sterlitech
PETEDD14225
POLYESTER(PETE)DRAINDISCS,142MM,25/PK
chrono-log
386
Thrombin凝血酶
Epigentek
E24009-1
HDAC6Protein
mybiosource
MBS844519
HDAC6,RecombinantProtein
InnovativeResearch
IPLA-SER
PooledNormalHumanSerum
ARR-002-50ul
Anti-RyanodineReceptor2
R-500-5mg
Ryanodine
schircks
11.483
(6R,S)-6-Methyl-5,6,7,8-tetrahydropterindihydrochloride
11.506
6,7-Dimethyl-7,8-dihydropterinmonohydrochloride
13.206
6-Hydroxy-7,8-dihydrolumazine
11.260
Oncopterin
11.480
6-Methylpterin
11.481
6-Methyl-7,8-dihydropterinhydrochloride
艾本德Eppendorf
3122000051
bio-rad
1708235
OPTI-4CNSUBSTRATEKIT
scicominc
BB31695-PE/8
PE-190导管，MedicalGradeMicroTubing,CoiledMetricLowDensity,0.047x0.067,1.19x1.70
07306-15
PolybeadMicrospheres0.35m
Axis-Shield
AS1002424
Nycodenz粉剂
Abbexa2017
abx125006
Acetyl-TP53-K370Antibody
abx055061
RatYKL-40/CHI3L1ELISAKit
Chromogenix
82190063
COASETFVII
tocris
1045
CNQXdisodiumsalt
1128
Picrotoxin
1078
Tetrodotoxin(1MG)
Tocris
0106
D-AP5(10MG)
Hitobiotec
HTKNS1125NH
HITOGOLGI-COXOPTIMSTAINPREKIT
SageScience
KBB1001
KBBGEL,0.5XKBB
tedpella
15717
ColdWaterFishGelatin,45%
0082085239
S-228825mg
dyets
510017
L-AminoAcidDefinedAIN-93GDiet
cygnus
F410
LS002294
Elastase,Lyophilized
H-2496.0005
Ac-Asp-Glu-Val-Asp-aldehyde(pseudoacid)
cmctag
AT0014
anti-YeastbetaActinmousemAb
Ubiquitin-ProteasomeBiotechnologies
J1100
E2ScreeningKit
M-2615.0005
(Z-Asp-Glu-Val-Asp)-Rhodamine110
M-2610.0005
AMCA-Glu-Glu-Lys-Pro-Ile-Ser-Phe-Phe-Arg-Leu-Gly-Lys(biotinyl)-NH
P10945-BdEO
Poly(butadiene(1,2addition)-b-ethyleneoxide)
avanti
840875P
18:1PA
DenvilleScientificInc
M0700(1002543)
Smallroundcoverglass,#1thickness,5mm
F500
VeroCellHCPELISAkit
ENZO
BML-EI321-0010
TOFA
MRC-Holland
P100-025R
SALSAMLPAP100MYBPC3probemix25rxn
P196-025R
SALSAMLPAP196TNNT2-BAG3probemix25rxn
P418-025R
SALSAMLPAP418MYH7probemix25rxn
novus
NBP2-14024
RabbitPolyclonalIrisin/FNDC5Antibody
5000006
PROTEINASSAYDYERGNT,450ML
r-biopharm
P1001
chromadex
ISL-10040006-001
CDNIsotopes
D-4093
(RS)-S-Adenosyl-L-methionine-d3(S-methyl-d3)tetra(p-Toluenesulfonate)Salt
10139092035
L-GlutamicAcid
16040-30
FastDryingSilverPaint,30g
corning
354115
Corning:emoji:T-CellCultureSupplementwithConA(IL-2CultureSupplement)
GOLDBIO
LUCK-1G
D-荧光素钾盐(cas:115144-35-9)
athensresearch
16-16-A32001
Apotransferrin,HumanPlasma
BioXcell
BP0101-100mg
InVivoPlusanti-mousePD-L1(B7-H1)
promocell
C-25010
HepatocyteGrowthMedium(Ready-to-use)
TriLink
N-2059-1
5-Hydroxymethyl-2-deoxyuridine-5-Triphosphate
品牌bioaustralis
BIA-C1208
CyclosporinA/环孢菌素A，CAS号：59865-13-3
microspheres-nanospheres
790120-010
GoldNanospheres，Size,[nm]80
InGexInfo
TGIRT5x50
TGIRT-IIIEnzyme
nordicbiosite
EKU5154
MDA
biokits
BQ096D
CotinineELISAKit,Single
life
AM9261
EDTA(0.5M),pH8.0
IPLA-N
PooledNormalHumanPlasma
IRA
SE-121-0.25mg
17-ESTRADIOL60Days
21759-1
NylonWoolFiber,Syringe(GammaIrradiated)
18369-50
NylonWoolFiber
18369-10
LS004197
LS004194
Collagenase,Type1
lumiprobe
67320
Sulfo-Cyanine5.5NHSester
Lumigen
TMA-100
LumigenECLUltra
Cayman
18113
YM-53601
SignalChem
E10-11G-05
ActiveKinases
E10-11G-10
biospacific
J46820
ZikaEnvelopeAntigen,Recombinant
BiosPacific
A37130
IgM
QIAGEN
12362
EndoFreePlasmidMaxiKit(10)
laysanbio
DSPE-PEG-MAL-2000-1g
DSPE-PEG-MAL-5000-1g
CordenPharma
LP-R4-117
DOTAPCl
LP-R4-069
DOPE
LP-R4-070
DOPC
LP-R4-124
POPG,Na
merck
324674-1MGCN
EGFRInhibitor
324833-5MGCN
EGFRInhibitorIII*TKS050*
521232-1MGCN
PDGFRTyrosineKinaseInhibitorIII
521233-1MGCN
PDGFRTyrosineKinaseInhibitorIV
se-121-0.72mg
simicon
SPW8103-9-E
Bacillusatrophaeusinaquadest.
SPE8103-9-E
CSTTechnoligies,Inc
ss2105-1000
SeraSub
US2205-1000
UriSub
SRM3132
Manganese(Mn)StandardSolution
SRM3129a
Lithium(Li)StandardSolution
SRM3149
Selenium(Se)StandardSolution
MBS1394376
InterferontypeB(IFNB),RecombinantProtein
CellSciences,Inc.
CM001
ReplacedbyCRI500(RecombinantHumanInsulin-likeGrowthFactor1,ReceptorGrade)
FRONTIERINSTITUTECo.Ltd
A2A-Go-Af700
A2Aadenosinereceptor
cisbio
62HIL1BPEH
chondrex
7001
CompleteFreundsAdjuvant,4mg/ml
20021
ImmunizationGradeBovineTypeIICollagen
sysy2017
139103
VAChT
Avanti
610014
FilterSupport|610014，10mmFilterSupports(100/pk)
86057-10
BioMagPlusConcanavalinA
Biomatik2017
CAU21277
T-CellImmunoreceptorWithIgAndITIMDomainsProtein(TIGIT)PolyclonalAntibody
Nanoprobes,Inc
111
AuroVist15nm5vials
7316214
AG50W-X82-4HIN.8X4COL,50
pel-freez
41052-2
RABBITBRAINCEPHALINEXTRACT(10ML)
1432445
AG1X8,100-200,BT,OH,100G
PhytoTech
K378
Kanamycin
Dharmacon
M-019759-00-0005
EchelonBiosciences
Q-0013-1mg
Inositol1,3-bisphosphate(Ins(1,3)P2)
P-3004-1mg
Phosphatidylinositol3-phosphatediC4(PI(3)PdiC4)
PA03001
PA03N0.500μm
PK401
IMCOCorporationLtdAB
TRX-01-B
Thioredoxin1,E.coli
TR-01
Thioredoxinreductase,E.coli
27104
QIAprepSpinMiniprepKit(50)
AGPHARMACEUTICAL,INC
AG10006
Anti-MAP1S
Cellsignaling
8954S
Phospho-Tyrosine(P-Tyr-1000)MultiMabRabbitmAbmix
MerckMillipore
16-638
Anti-Phosphotyrosine4G10Platinum,
682404
AlexaFluor647anti-HA.11EpitopeTag
321132
AlexaFluor700anti-humanCD206(MMR)
141720
PE/Cy7anti-mouseCD206(MMR)
302216
PE/Cy7anti-humanCD19
141605
APCanti-mouseH-2KbboundtoSIINFEKL
eBioscience
56-0032-82
Anti-MouseCD3AlexaFluor700100ug
viagenbiotech
21141
NucleicAcidStainingSolution
medicago
10-0027-1
ProteinLLigandLeakageELISA
Associatesofcapecod
GT003
GlucalKit
proliantbiologicals
68700
ReagentGrade(FattyAcid-Free)
MBS2018594
Gastrin(GT),RecombinantProtein
BE0001-2-50MG
InVivoMabanti-humanCD3
LifeSpan
LS-B2606-25
Anti-PITX3Antibody(N-Terminus)IHC-plusLS-B2606
UpchurchScientific
p-675
1/4-28FemaletoMaleLuerAssy（外螺纹Luer到1/4-28外螺纹转接头(红色，ETFE)Luer到1/4-28个）
p-678
LuerAdapter1/4-28FemaletoFemaleLuer,Tefzel(ETFE)9内螺纹Luer到1/4-28内螺纹转接头(红色，ETFE)Luer到1/4-28)
avivasysbio
ARP39385_T100
SRM2783
AirParticulateonFilterMedia
nibsc
10/286
FollicleStimulatingHormone,LuteinizingHormonehuman,urinaryforbioassay(5thInternationalStandard)
Roboz
RS-4925
CeramicCoatedDumont#5ForcepsInoxTipSize.05X.01mmBiologieTips
RS-4955
Dumont#5SFSuperFineForcepsInoxTipSize.025X.005mm
RS-5001
Dumont#5ForcepsStainlessSteelHalfCurveTipSize.02X.06mmBiologieTips
RS-5002
Dumont#5ForcepsNewShapeInox.05X.01mmBiologieTips
RS-5005
Dumont#5Forceps45DegreeInoxTipSize.05X.01mmBiologieTips
RS-5039
DumontTweezers;Pattern#7;.07X.03mmTipSize;115mmLength;Material:Titanium;BiologieTips
RS-6416
RS-6417
RS-6561
RS-5431
MicroClipSlightCurve1X6mm
RS-5433
MicroClipStraightStrongCurved1X6mm
RS-5422
MicroClipStraight.75X6mm
RS-5440
RS-5831
RS-5830
RS-9911
2.5LUltrasonicSurgicalInstrumentCleaner
RS-300
GeminiCauterySystem
RS-301
5mmGeminiDisposableCauteryTips;PackOf10
RS-302
10mmGeminiDisposableCauteryTips,Packageof10
RS-5640
ExtraFineVannas3.5(8.5cm)Long,2mmX0.05mmCuttingEdge(LengthXTipWidth),Straight
RS-5641
ExtraFineVannas3.5(8.5cm)Long,2mmX0.05mmCuttingEdge(LengthXTipWidth),Curved
RS-5605
cPherson-Vannas3(7.5cm)Long3X0.1mm(CuttingEdgeLengthXTipWidth)Curved
RS-5807PencilHandleScissors
RS-5805PencilHandleScissors
RS-4978
Dumont#5ForcepsDumostarTipSize.05X.01mmBiologieTips
RS-4984
Dumont#55ForcepsDumostarLightThinTipsSize.05X.01mmBiologieTips
RS-4919
Dumont#5Forceps90DegreeStainlessSteelTipSize.05X.01mmBiologieTips
RS-5058
Dumont#5Forceps45DegDumoxelTipSize.10X.06mm
RS-6200
MicroLigatureNeedle4.75inBluntLeft
RS-6202
MicroLigatureNeedleLeftcurve
RS-6204
RS-7113
ML-643
LoupesOnGlasses,5X420mmworkingdistance
ML-605
LEDLightForLoupes
ML-692
Clip-onCliptoattachLEDlighttoloupes
ML-693
Slide-oncliptoattachLEDlighttoloupes
RS-9900A
RS-5138GraefeForceps
4(10cm)LongFullCurveExtraDelicate0.5mmTip
RS-7231
RS-8614
RS-5135GraefeForceps
4(10cm)LongSerratedSlightCurve0.8mmTip
RS-5130GraefeForceps
4(10cm)LongSerratedStraight0.8mmTip
RS-5882
RS-6240
JBS-CS-203L
JBScreenBasicHTS
JBS-CS-201L
JBScreenClassicHTSI
JBS-CS-202L
JBScreenClassicHTSII
343606
PEanti-humanCD34
F280
InsulinELISAkit,Ultra-Sensitive
F551C
Anti-CHO:HRPConjugateConcentrate,3G
F550
CHOHCPELISAkit,3G
IF0001
ProteinA/GAgaroseBeads
Biorelevant
02131101-25L
FaSSIF,FeSSIFFaSSGFPowder(SIFPowderOriginal)
1910
Oxytocin
InventBiotechnologies,Inc.
AT-022
MinuteTotalProteinExtractionKitforAdiposeTissues/CulturedAdipocytes
28604
MinEluteGelExtractionKit(50)
28004
MinElutePCRPurificationKit(50)
ActiveMotif
39925
HistoneH4ac(pan-acetyl)antibody(pAb)
Lumiprobe
A1330
Sulfo-Cyanine3azide,1mg
A3330
Sulfo-Cyanine5azide,1mg
PROGEN
GP62-C
anti-p62/SQSTM1(C-terminus)guineapigpolyclonal,serum
61038
anti-KeratinK8mousemonoclonal,Ks8.7,lyophilized,purified
61028
monoclonal,Ks18.04,lyophilized,purified
61017
anti-Clathrinmousemonoclonal,CHC5.9,lyophilized,purified
phytotech
M519
MSBASALMEDIUMw/VITAMINS
megazyme
K-ACETGK
AceticAcidGKAssayKit(AnalyserFormat)
IRMM欧洲标准局
BCR-656
ISOTOPERATIOSINABSOLUTEALCOHOL25,
BCR-660
ISOTOPERATIOSINALCOHOLICSOLUTION26,
QA-Bio
E-BG07
Beta-(1-4)-Galactosidase,E-BG07
E-BG02
Beta-(1-3,4,6)-Galactosidase,E-BG02
E-F134
Alpha-(1-3,4)-Fucosidase,E-F134
QA-Bio2017
E-F006
Alpha-(1-6)-Fucosidase,E-F006
cellsciences
CRC168B
RecombinantHumanCrystallinAlphaB
biorelevant
FaSSIF/FeSSIF/FaSSGFFFF03
MaiiaDiagnostics
Art.No.0070
Anti-hEPO,3F6,1mg
Art.No.0080
Anti-hEPO,7D3,1mg
Art.No.1390
EPOPurificationKit
bbottLaboratories
02J01-036
PathVysionHER-2DNAProbeKit(IVD)GTIN：00884999001763
AATBioquest
22200
JC-1[5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanineiodide]*CAS#:3520-43-2*
15204
DCFH-DA[2,7-Dichlorodihydrofluoresceindiacetate]*CAS4091-99-0*
17-0049-42
Anti-HumanCD4APC100tests100tests
southernbiotech
2049-01
GoatAnti-HumanIgG,Monkeyads-UNLB
mrc-holland
P140-025R
SALSAMLPAP140HBAprobemix25rxn
P102-025R
SALSAMLPAP102HBBprobemix25rxn
TRC
E588688
Epirubicin
Anti-HumanCD4APC100tests
12-0049-80
Anti-HumanCD4PE25ug
45-0049-41
Anti-HumanCD4PerCP-Cyanine5.525tests
17-0088-41
Anti-HumanCD8aAPC25tests
45-0088-41
Anti-HumanCD8aPerCP-Cyanine5.525tests
12-0088-41
Anti-HumanCD8aPE25tests
00-6993-50
7-AADViabilityStainingSolution2mL
14-9832-82
Anti-MouseCaspase1Purified100ug
45-0289-41
Anti-HumanCD28PerCP-Cyanine5.525tests
17-0279-41
Anti-HumanCD27APC25tests
GenWay
GWB-MT533B
HADHAntibody
MBS422492
IDH2,PolyclonalAntibody
M7514(M-7514)
52304
QIAampRNABloodMiniKit(50)
HyTest
2I1
Monoclonalmouseanti-humaninsulin,MAb:C7C9
BioAssaySystems
E2GN-100
EnzyChromGlycogenAssayKit
RD
DCMP0
HumanCOMPQuantikineELISAKit(1KT)
Cytoskeleton
PHDR1
RhodaminePhalloidin(14uMinmethanol)
cerilliant
D-907
Diazepam,1.0mg/mL
c-022
Chlordiazepoxide,1.0mg/mL
MBS143105
ComplementComponentC1q,NativeProtein
mbs320188
Norovirus,MonoclonalAntibody
CrystalChem
80300
RatHemoglobinA1c(HbA1c)AssayKit
dyenamo
DN-P02
MethylammoniumiodideCH3NH3I
DN-P04
Cobalt-basedp-typedopantfororganicholeconductors(FK209)
DN-P08
MethylammoniumchlorideCH3NH3Cl
DN-P09
MethylammoniumbromideCH3NH3Br
DN-P10
Formamidiniumiodide,Formamidinehydroiodide
DN-P11
Formamidiniumbromide,Formamidinehydrobromide
DN-EVEA01
EversolarSpiro-OMeTADcas：207739-72-8
dyesol
MS101000-50
Methylammoniumiodidecas#14965-49-2
MS301000-50
MethylammoniumbromideCAS#6876-37-5
MS220305-05
FK209Co(III)TFSIsaltCAS#1447938-61-5
MS002300-100
30NR-D
MS002010-100
18NR-TTransparentTitaniaPaste
MS150000-10
Formamidiniumiodide
MS350000-10
Formamidiniumbromide
MS002500-20
BL-1BlockingLayer
16447500
FreeStyleMAXReagent
bioreclamationivt
Z990012
InVitroGROHIMedium
Z99009
bioaustralis
BIA-Z1405
-Zearalanol
INCELLCorporation
M300F-500
CULTUREMEDIAM3:BaseF:trademark
PK-6102
VECTASTAINEliteABC-PeroxidaseKit
quantabio
95107-100
qScriptmicroRNAcDNASynthesisKit
phytotechlab
MurashigeSkoogBasalMediumwithVitamins
M524
MurashigeSkoogBasalSaltMixture
A111
Agar,MicropropagationGrade
MBS826733
ProteinAGAgaroseIP,Reagent
selleck
S1228
IdarubicinHCl
STRECK
218962
Cell-FreeDNABCT（基因无创管）
E-305-025
RecombinantHumanUbiquitinActivatingEnzyme(UBE1),CF(25UG)
E2-622-100
RecombinantHumanUbcH5b/UBE2D2Protein,CF(100UG)
U-110-01M
RecombinantHumanHA-UbiquitinProtein,CF(1MG)
enzymatics
P7010-HC-L
Klenow(35exo-)(HighConcentration)
transgenic
KO604
AntiHumanKlothoMonoclonalAntibody(CloneNo.KM2119)
4342792
TempusBloodRNATube
LS-C253424-200
Anti-CASP3/Caspase3Antibody(aa155-185,APC)
S03316
InVitroGROCPMedium,serum-free(Note:add25mLofFBSwheninuse)
FAB1418A
HumanGlut1AllophycocyaninMAb(Clone202915)(100TESTS)
Corning
354480
CorningBioCoatMatrigelInvasionChamberswith8.0mPETMembraneintwo24WellPlates,12/Pack,24/Case
irvinescientific
9988
HumanSerumAlbuminSolution
PolyScientific
s2364
GoldChloride0.5%Aqueous
teknova
P1110
PichiaDropOutAdenineSelectiveAgarPlates100mm.Sterile
CarnaBiosciences
05-084
IKK(IKBKB)
MBS2028466
Interleukin18(IL18),PolyclonalAntibody
SierraBioresearch
Gemcitabine-5-triphosphate(dFdCTP)
vistalab
1065-10mL
MBS2002408
Interleukin1Beta(IL1b),PolyclonalAntibody
LKTLaboratories
C9613
CYCLOSPORINDCasNo.63775-96-2
seracare（老品牌kpl)
5230-0307
Anti-MouseIgG(H+L)Antibody,HumanSerumAdsorbedandFITC-Labeled（老货号02-18-06）
OneLambda
CABC-1D
ClassIComplement
ImmuneSystemsLtd
BVS100
FastReadCountingSlides(100slides/box)
BD
610182
E-CadherinPure36/E-Cadherin150ug
immunodx
1081
HIV-1ADAgp120
RamcoLaboratories,Inc.
S-22
SPECTROFERRITINMicroplateAssayKit
molecularDevices
K8220
CloneDetectSpcifiquedelIgG(H+L)desouris,fluorescine
K8610
CloneMediaSemi-SolidMediaforHybridomaandMyelomacells,90ml
K8865
CloneMediaHybridomaSemi-SolidSelectionandCloningMedium
K8867
CloneMediaHybridomaSemi-SolidSelectionandCloningMedium(withoutHAT)
MBS1354582
Homogentisate1,2-dioxygenase(HGD),RecombinantProtein
MBS452438
Lipopolysaccharides(LPS),ELISAKit
zenoaq
CELLBANKER2
wpiinc
TW100F-6
ThinWallGlassCapillaries
LS-F14141-1
MouseB2M/Beta2MicroglobulinELISAKit(SandwichELISA)
AVIVASYSTEMSBIOLOGY
OKEH00344
B2MELISAKit(Mouse)
bethyl
E101-117
PigIgMELISAKit
E101-102
PigIgAELISAKit
E101-104
PigIgGELISAKit
Bachem
H-7404.1000
Amyloid-Protein(1-42)sodiumsalt
FAPAS
T2717QC
FCAL16-CON2QCPeanutinChocolateQualityControlMaterialTestMaterial
T27168BQC
FCAL6-CCP37QCGluteninOatBasedFoodstuffQualityControlMaterial
T27162BQC
FCAL8-CON2QCHazelnutinChocolateQualityControlMaterialTestMaterial
1613108
CERTAGAROSE,MEGABASE,25G
1613109
CERTAGAROSE,MEGABASE,125G
1613110
CERTAGAROSE,MEGABASE,500G
Bio-Rad(AbDSerotec)
MCA1218F
MOUSEANTIPIGCD14:FITC
MCA1751PE
MOUSEANTIPIGCD45RA:RPE
KamiyaBiomedicalCompany
KT-32057
Beta2Microglobulin(b2M)ELISAKit,Mouse
430904
MouseTNF-ELISAMAXDeluxe
127510
AlexaFluor647anti-mouseCD47
100725
PacificBlueanti-mouseCD8a
100220
PE/Cy7anti-mouseCD3
NBP2-32473
RabbitPolyclonalC14orf39Antibody
900400P
Palmiticacid(15-yne)
GraceBio-Labs
112358
CS16-CultureWellwithRemovableChamberedCoverglass,16Well,No.1.5DESAGCoverglass,STERILE8PACK
GP40
anti-Perilipin2(N-terminusaa1-29)guineapigpolyclonal,serum
sysy
287006
chickenpolyclonal,purifiedIgY
BE0188-5mg
InVivoMabanti-humanPD-1(CD279)
InventBiotechnologies
SM-005
MinutePlasmaMembraneProteinIsolationKit
PROMEGA
A1125
DNAGENOTEK
PT-L2P-1.5
prepITL2P
abbexa
abx350204
RatReninELISAKit
fishersci
23-295-695
Pro-LabDiagnosticsMicrobankAccessoryProducts
Chiralix
CX50506-10mg
6-phenoxy-1-phenyl-2-(1H-1,2,4-triazol-1-yl)hexan-1-one
STRATECBiomedicalAG
1038111200
StoolCollectionTubeswithStoolDNAStabilizer
KE-SIALIQ
SialicAcidQuantitationKit
clodronaiposomes2017
78clp005ml
ClodronateLiposomesandPBSLiposomes
16074
CopperConductiveTape,DoubleSidedAdhesive,12.7mmWx16.4mL
16034
PELCOColloidalSilver,15g
16023
Acetone,Thinner/Extender/Cleaner,30ml
16031
PELCOColloidalSilver,30g
285-IF-100
RecombinantHumanIFN-gammaProtein(100UG)
CM-PEG-DSPE-5000-500mg
G523
DL-PhosphinothricinSolution(1mg/mL)
listbiologicallaboratories
171E
AnthraxProtectiveAntigen(PA),RecombinantfromB.anthracis
ENZ-51042-K500
ROS-IDHypoxia/Oxidativestressdetectionkit
ENZ-51042-0125
abbkine
ABP53254
Glut4PolyclonalAntibody
BE0089-5MG
InVivoMabratIgG2aisotypecontrol
MBS8242688
IL-1F10PolyclonalAntibody
IrvineScientific
90129
90128
lucigen
60502-2
TG1ElectrocompetentBacterialCells
17150-10
FluoresbriteYGMicrospheres0.10μm
17156-2
FluoresbriteYGMicrospheres6.00μm
MedKooBiosciences
522419
BBIsolution
BM322-M4E5
TroponinImousemonoclonalantibody
BM322-N4D1
TroponinImonoclonalantibody
cellscript
C-MSC100625
T7mScriptStandardmRNAProductionSystem
Helios
HPCFDCGL50
UltraGRO-Advanced,(GMPGrade)
AgenaBioscience,Inc.
10158
COMPLETEiPLEXGOLDGENOTYPINGREAGENTSETS
G300
GilderGrids,300mesh,3.05mmO.D.,CenterandRim-Mark,Copper
ALX-804-439-R100
Folatereceptormonoclonalantibody(Mov18)
BiosearchTechnologies
G-1000-10
N1-Guanyl-1,7-diaminoheptane(GC7)
G-1000-100
ImmunoChemistryTechnologies,LLC
649
GeneralSerumDiluent
organotechnie
19553
胰蛋白胨N1，TryptoneN1
angiobio
11-034
anti-mouseLYVE-1
macrocyclics
B-280
DOTA-NHS-ester
GeneBridges
K002
CounterSelectionBACModificationKit
AS1114544
Lymphoprep分离液(即用型)
AmericanBiochemicalPharmaceuticalsLimited
ABP-COL-2
COLLAGEN(EQUINETENDONTYPEI)
LS004182
Collagenase,Type3
305020
BrilliantViolet421anti-humanCD64
therapak
10300E
76mmx102mmZorbSheet，48mL
10314E
4Bay,Aqui-PakAbsorbentPak150mL
ALZET
1004
Chromsystems
0071
HomocysteinePlasmaControl,Bi-Level(I+II)
0072
HomocysteinePlasmaControl,LevelI
0073
HomocysteinePlasmaControl,LevelII
F70101C-A-2
F70101C-A-10
rpeptide
A-1119-1
Fluorescein-Beta-Amyloid(1-42)
abx250308
HumanCRHELISAKit
MBS652918
RPMI1640MediumModifiedw/oL-Glutamine,w/oAminoacids,Glucose,CultureMedia
LaborDiagnostikaNord
BAE-4200
NoradrenalinePlasmaELISAHighSensitive
ldn
BAE-5200
NoradrenalineResearchELISA
Pel-Freez
31061-3
3-4WEEKBABYRABBITCOMPLEMENTSTERILE(3-4周龄兔补体无菌)
ALPCO
74-TXBHU-E01
TXB2ELISA
14059
S18886
16845-100
Polyetherimide
PHCC60050
ScepterSensors,60uM,50PK
880126P
DSPE-PEG(2000)Maleimide
fdneurotech
PO101
Gelatin-CoatedMicroscopeSlides
CST
3671T
Phospho-MyosinLightChain2(Ser19)Antibody
3671S
lifesensors
UM401
Anti-UbiquitinTUBEs(non-selective)
MBS733114
alphaSmoothMuscleActin(alpha-SMA),ELISAKit
dianova
DIA-310
Anti-CD31(Ms,FFPE)fromRat(Clone:SZ31)
Cedarlane
FL1S
FLAER(Alexa488proaerolysinvariant)liquidformat-ASR
AvivaSystemsBiology
OABB01713
101-T
DirectPCRLysisReagent(MouseTail)
102-T
Iduron
CD003
UAGalNAc,6S
CD004
UAGalNAc,4S,6S(diE)
CD005
UA,2SGalNAc,4S(diB)
DirectPCRLysisReagent(MouseTail)100ml
agdia
PSA39300/0096
TomatoSpottedWiltVirus(番茄斑点枯萎病毒)
PSA64001/0096
SRA24100/0500
SouthernBeanMosaicVirus(南方菜豆花叶病毒)
17134
OCH
ClementeAssociates
gam250hl
goatantiMouseIgG[H+Lchains]
LS002138
CellGuidanceSystems
GFH6-1000
RecombinantHumanActivinA
PLasmidfactory
PF1160
pEPito-hCMV/EF1-EGFPLuc
Beckman
N-1510-100
Adenosine-5-Triphosphate
356904
PEanti-humanCD185(CXCR5)
bioline
BIO-21127
MyTaqExtract-PCRKit
abnova
H00050848-M01
F11Rmonoclonalantibody(M01),clone2E3-1C8
spherotech
FSVM-02556-2
StreptavidinCoatedFluorescentMagneticParticles(NileRed)
LS-F8064-1
HumanFNDC5/IrisinELISAKit(SandwichELISA)
MP-182
PrecisionTrochar
MBS616509
FOXD4L2(FOXD4L3),PolyclonalAntibody
AthensResearchTechnology
16-16-090707-FC
ImmunoglobulinG,FCFragment,HumanPlasma
ACC-017-0.2ml
Anti-TRPC6
indoor
EL-DF1
Derf1ELISA-10
EL-BG1
Blag1ELISA-10
EL-FD1
Feld1ELISA-10(6F9/3E4)
EL-CF1
Canf1ELISA-10
EL-AA1
Alta1ELISA-10(2C10/3B6)
eaglebio
DCM074
S100BELISAAssayKit
4364340
TaqManUniversalPCRMasterMix
10821015
10bpDNALadder
Rockland
601-1302
BovineIgG(HL)AntibodyPeroxidaseConjugated
wako
300-93523
Phos-tagAcrylamideAAL-107
0082141339
S-276525mg
0082032439
S-223825mg
A28-18G-05
AURORAA,Active
A28-18G-10
Biomatik
EKU10059
HumanAngiotensin1-7(Ang1-7)ELISAKit
Biovendor
RD192090100R
S100B
LCLaboratories
R5000
雷帕霉素Rapamycin
M39-10BG-05
p38alpha,Active
M39-10BG-10
AM9738
TRIReagentSolution
U232-380G-20
UBE2O,Active
U232-380G-50
18340-5
FluoresbriteBBCarboxylateMicrospheres4.50μm
19102-2
FluoresbriteBBCarboxylateMicrospheres6.00μm
signosis
CS-1001
TFPathwayProfilingCellArray
CS-1002
formedium
PMD0401
EMMBrothwithoutDextrose
PMD0402
PMD0201
EMMBroth
上海金畔生物科技有限公司代理AATBioquest荧光染料全线产品，欢迎访问AATBioquest荧光染料官网了解更多信息。
简要概述
用于定量测定血清/血浆样品和细胞培养上清液中的人类载脂蛋白A1（apoA1）。请注意，洗涤，封闭和孵育缓冲液应包含清洁剂。吐温20，TritonX-100或NP40的使用浓度为0.05-0.5％。在封闭缓冲液和孵育缓冲液中，建议使用0.1％BSA，而不要使用牛血清，因为HDL44也可以结合牛apoA1。
试剂：抗体是在含有叠氮化钠（0.02％）的无菌过滤（0.2μm）PBS中提供的。链霉亲和素-ALP用0.1MTris缓冲液和0.002％KathonCG提供。标准范围：0.6-40ng/ml
产品说明书
样品实验方案
储备溶液配制
通过将小瓶4内试剂重新溶于含1％BSA的1mlPBS中来制备apoA1标准品，不要搅拌，在室温下放置15分钟，然后涡旋振荡3s。得到的储备液为4μg/ml，应立即使用或以等分试样的形式储存在-20°C下，以备将来使用。我们建议等分试样在初次使用后不要重新冷冻。为了进行测试，请使用标准范围作为指导来准备原液的稀释液。
操作步骤
APExBIO品牌代理
APExBIO（APExBIOTechnologyLLC）总部位于美国休斯顿，是一家自主研发生产、致力于为生命科学研究提供高性能产品和优质服务的供应商。公司现阶段涵盖丰富的产品线，主要包括：
1.10000多种小分子抑制剂/激动剂——用于肿瘤、免疫、神经等生物医药各研究领域
2.40余个高通量化合物库——药物筛选最佳工具
4.体外转录——公司拥有国内D家mRNA体外转录合成平台，提供优质的修饰核苷酸、转录酶等各种合成原料，并可提供mRNA订制服务
5.Cy染料、生物素——高纯度、种类丰富的Cy染料和生物素可用于蛋白检测、纯化等研究
6.免疫学产品——各类细胞器染料及靶标信号放大的链霉亲和素系列和TSA系列
7.试剂盒——高性能的MTT、CCK-8、EdU细胞增殖与毒性检测Kit和AnnexinV、Caspase凋亡检测Kit
8.蛋白研究——蛋白提取抑制剂cocktail、磷酸化蛋白研究新工具phosbind
9.组学服务——提供微生物多样性、转录组学等高通量测序服务
APExBIO常卖产品：
Customdietsaredevelopedforaspecificpurposeandbenefitfromyourinputandourexpertise
Determiningthemostappropriatedietforyourresearchmodelcanbechallenging,butweareheretohelp.Withmorethan20,000uniqueformulasinourdatabaseattainedover40years,Inotivnutritionistshavevastexperiencetodrawupon.
Tomeettheprogressivedemandsofscienceandtoserveyoubetter,wecontinuallyaddtoourdatabaseasweacquirenewknowledge.
Youcanselectfromthewidevarietyofexistingdiets,oronedesignedspecificallyforyourpurposes.Eitherway,weencourageyoutocontactusforcomplimentaryconsultationwithournutritionists.
Asmallnumberofrepresentativecustomresearchdietformulasarefoundonourwebsite.Ifyouareunabletofindaproductcodeorformuladescriptioncitedinapublication,oroneusedpreviouslybyyourlaborothers,contactus.
WantmoreinformationExpandthesectionsbelow,visitourorderingandservicespage,viewanswerstoourdietFAQs,orcontactusataskanutritionist@inotivco.com.
Commonclassesofpurifieddietsinclude:
YoucanuseoneofourcompletestandardnaturalingredientdietsfromeitherourGlobalorTraditionallinestoserveasthebaseforacustomdiet.Wecanaddvarioustypesofin-houseingredientssuchasdoxycycline,tamoxifen,cuprizone,cholesterol,lithium,carbonyliron,vitaminE,aspirin,ibuprofen,andmore.Wealsoroutinelyaddcustomer-suppliedingredients.
Customnaturalingredientdietsareformulatedusingthesameagriculturalcommoditiesasfoundinourstandardlaboratoryanimaldiets.Theseincludecorn,wheat,soybeanmeal,alfalfameal,fishmeal,andotheragriculturalby-products.Theseingredientshaverelativelycomplexandvariablechemicalcomposition,andthustheiruseincustomdietsislimited.TheNaCladjustedseriesforrodentsareoneexampleofcustomdietsinthiscategory.
Hybriddietsareamixtureofnaturalandrefinedingredients.Thistypeofblendedformulationissometimesnecessarytolimitspecificnutrientornon-nutrientsubstances.Theinclusionofsomenaturalingredientscanenhancepalatabilityofahybriddiet,particularlyforrabbitsandguineapigs.
Youmayhaveaspecificadditivethatyouwouldlikeustoincludeinoneofourpurified,standard,naturalingredient,orhybriddiets.Uponreviewofsafetyinformation,wecandothatforyou.Itisoftenaconvenientapproachfordosingresearchanimals.Someoftheadditivesyoumayconsiderincludefoodextracts,antioxidants,anti-inflammatoryagents,fattyacids,pharmaceuticaldrugs,andmanymore.Seecustomer-suppliedingredientstolearnmore.
jenabioscience品牌代理
JenaBioscience由马克斯-普朗克分子生理研究所（Dortmund）的科学家团队于1998年成立，利用超过25年的专业知识为来自100多个国家的研究和行业客户开发创新试剂。迄今为止，JenaBioscience仍然是一家所有者经营的企业。JenaBioscience由德国马普所（MPI）的分子生理学家1998年共同创办的生物公司，致力为全球的科研院所、医院、制药企业以及诊断试剂盒生产企业提供各类试剂。在结构学研究方面提供晶体筛选试剂盒、优化试剂、工具和各类耗材等。
jenabioscience常卖产品：
日本新田明胶低内毒素胶原蛋白,Bematrix胶原蛋白,NittaGelatin
碱法猪皮明胶胶冻强度高
内毒素减少的标准明胶
猪皮碱加工明胶胶冻强度低
内毒素减少、安全性提高的明胶水解物
储存条件：室温
有效期：自生产之日起2年
-浓度：0.1%
胃蛋白酶溶解的猪皮胶原蛋白
储存条件:冷冻储存
●浓度：5mg/ml
胶原蛋白是一种存在于多细胞动物细胞外的蛋白质，可以用酸或酶提取。明胶通过加热胶原蛋白而变性，通过酶或高温处理降低明胶的分子量，制成胶原蛋白肽或明胶水解物。明胶和胶原蛋白肽在性质上的主要区别在于胶凝能力的存在与否。
猪腱酸提胶原蛋白液
内毒素水平：0.5EU/ml以下
储存条件：冷藏（4°C至8°C）
●浓度：3mg/ml[pH3]
猪皮胃蛋白酶溶解胶原蛋白溶液
浓度:5mg/ml[pH3]
来自猪皮的冻干去端肽胶原
可配制高浓度胶原蛋白溶液
内毒素水平：100EU/g以下
●产品外观：白色海绵
-尺寸：约140×100×10mm
●应用
CellmatrixTypeI-A是针对3D培养的胶凝速度和透明度进行了优化的产品。在细胞沉入培养容器底部之前可以快速形成凝胶。此外，凝胶具有极高的透明度，便于观察细胞。
这是一种在涂有胶原蛋白分子的培养基质上培养细胞的方法。它有望通过与胶原蛋白分子接触来改善细胞粘附并激活细胞内信号。
推荐产品：CellmatrixTypeI-C、CellmatrixTypeI-P
每种Cellmatrix产品的胶凝能力和粘度各不相同。使用胶原蛋白凝胶培养时，请使用凝胶化能力优良的产品。此外，产品粘性越小，越容易处理，适合包被培养。下面总结了每种产品的特点，供您参考。
MilliporeA10紫外灯ZFA10UVM1
A10紫外灯适用于A10TOC检测仪，测量并控制溶解在超纯水中的痕量有机污染物。
适用Millipore机型：Milli-QA10Milli-QAdvantageA10Milli-QIntegral
moradec品牌代理
moradec常卖产品：
产品货号
钾（K+）离子通道在调节基本生物过程（包括心率，激素和神经递质分泌，水和电解质平衡）中起重要作用。钾离子通道已被认为是包括心律不齐，疼痛，糖尿病，神经功能障碍等在内的疾病适应症的药物靶标。Tl+通过K+通道的渗透性已被广泛用于测定K+通道。表达目的K+通道的细胞（例如hERG，Kv1.3，Kir2.1，KATP）预先装有Tl+敏感染料。该染料是非荧光的，并且对细胞膜是可渗透的。进入细胞内部后，非荧光AM酯染料会被内源性酯酶裂解为留在细胞内部的带负电荷的染料。将含有低剂量Tl+刺激缓冲液添加到细胞中时，Tl+会流过K+通道并与Tl+敏感染料结合，从而产生荧光信号。该信号与K+通道的活动成正比。如果将拮抗剂或拮抗剂添加至细胞，则荧光信号分别降低或升高，以反映K+通道的刺激性。金畔生物是AATBioquest的中国代理商，为您提供优质的ScreenQuest免洗钾离子通道检测试剂盒。
点击查看光谱
适用仪器
溶液配制
1.TI-520AM储备溶液：将20μL（#36550）或DMSO（#36551和＃36552）的DMSO加到TI-520AM（组分A）的小瓶中，并充分混合。注意：未使用的TI-520AM储备溶液可以等分并存储在<-20℃下。避光并避免重复的冻融循环。
2.分析缓冲液（1X）：将1mL的10XPluronicF127Plus（组分B）添加到9mL的HHBS缓冲液（组分C）中并充分混合。注意：10mL1X分析缓冲液足以用于一块板。分装并在<-20℃下存储未使用的10XPluronicF127Plus（组分B）。避光并避免重复的冻融循环。
3.无氯缓冲液（1X）：在8mL的ddH2O中加入2mL的无氯缓冲液（5X）（组分D），并充分混合。
4.Tl2SO4溶液（未提供，SigmaCat＃208191）：将Tl2SO4溶于超纯水中至终浓度80mM。注意：Tl2SO4有毒。采取必要的预防措施，以防止吸入和皮肤接触。
工作溶液配制
1.TI-520AM染料加载溶液：将20μLTI-520AM储备溶液（500X）加入10mL的1X分析缓冲液中并充分混合。
2.刺激溶液（5X）：在1X无氯化物缓冲液中用Tl2SO4稀释配体（用于非电压门控钾通道）或K2SO4（用于电压门控钾通道）。
表1.应针对每个靶标优化参考（HEK293-KCNH2细胞中的电压门控hERG通道），Tl2SO4和K2SO4（电压门控钾通道）或配体（非电压门控钾通道）的浓度及细胞类型。
组分
容积
5X浓度
浓度
无氯缓冲液(5X)
2mL
K2SO4(250mM)
1mL
25mM
5mM
TI2SO4(80mM)
0.5mL
4mM
0.8mM
ddH2O
5.5mL
总计:
10mL
实验步骤
1.将100μL/孔（96孔板）或25μL/孔（384孔板）的TI-520AM染料加载溶液添加到细胞板中。
2.将染料加载板在细胞培养箱中孵育1小时，然后在室温下再孵育15至30分钟。注意：如果测定需要37°C，请立即进行实验，而无需进一步室温孵育。
3.用HHBS准备K+通道拮抗剂或激动剂。
4.在细胞培养箱中用拮抗剂（用于抑制研究）将板孵育30分钟。
5.用FLIPR，FDSS或Flexstation加50X/孔（对于96孔板）或12.5μL/孔（对于384孔板）的5X刺激缓冲液。使用Ex/Em=490/525nm的滤光片组进行实验。每1-2秒读取板3分钟。
图示
图1.使用ScreenQuest钾离子通道试剂盒在HEK293-KCNH2细胞中测量了对hERG通道的剂量依赖性抑制。将细胞以20,000个细胞/100μL/孔在Costar黑色96孔板中接种过夜。将细胞与100μL染料加载溶液在37°C下孵育1小时。向细胞中加入10μL阿司咪唑，并在37°C下孵育30分钟。通过FlexStation将50μL含0.5mMTl2SO4和2.5mMK2SO4的刺激溶液注入每个孔中，并在激发/发射=490/525nm的情况下每秒钟读取3分钟。IC50=0.46μM。
图2.使用ScreenQuest钾离子通道试剂盒在HEK293-KCNH2细胞中测量了K2SO4剂量依赖性hERG通道活性。将细胞以20,000个细胞/100μL/孔在Costar黑色96孔板中接种过夜。将细胞与100μL染料加载溶液在37°C下孵育1小时。通过FlexStation将终浓度为1mM，0.5mM，0.25mM或0mM的Tl2SO4和不同浓度的含K2SO4的刺激溶液注入每个孔中，并在激发/发射=490/下每1-2秒读取平板3分钟。525nm。
产品基本信息
产品名称：PG-胆固醇胺
CAS：1264272-41-4
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：1043.73
外观：黄色固体
溶剂：DMSO
激发波长(nm)：498
发射波长(nm)：517
产品介绍
图2.PG-胆固醇胺的化学结构
参考文献
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产品名称：胆酸示踪探针CLF
分子量：926.12
InhibitionofbileacidtransportacrossNa+/taurocholatecotransportingpolypeptide(SLC10A1)andbilesaltexportpump(ABCB11)-coexpressingLLC-PK1cellsbycholestasis-inducingdrugsAuthors:MitaS,SuzukiH,AkitaH,HayashiH,OnukiR,HofmannAF,SugiyamaY.Journal:DrugMetabDispos(2006):1575
Oxidativestressinducesinternalizationofthebilesaltexportpump,Bsep,andbilesaltsecretoryfailureinisolatedrathepatocytecouplets:aroleforproteinkinaseCandpreventionbyproteinkinaseAAuthors:PerezLM,MilkiewiczP,EliasE,ColemanR,SanchezPozziEJ,RomaMG.Journal:ToxicolSci(2006):150
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Taurolithocholicacidexertscholestaticeffectsviaphosphatidylinositol3-kinase-dependentmechanismsinperfusedratliversandrathepatocytecoupletsAuthors:BeuersU,DenkGU,SorokaCJ,WimmerR,RustC,PaumgartnerG,BoyerJL.Journal:JBiolChem(2003):17810
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产品名称：葡萄糖转运/血糖通道荧光探针2-NBDG
CAS：186689-07-6
保质期：12个月
分子量：342.26
外观：红色固体
激发波长（nm）：485
发射波长（nm）：540
葡萄糖转运/血糖通道荧光探针2-NBDG是美国AATBioquest生产的用于检测葡萄糖/血糖的荧光探针。2-NBDG是荧光标记的脱氧葡萄糖类似物，主要用于直接监测活细胞和组织对葡萄糖的摄取。它还用作检测瘤形成的局部造影剂。2-NBDG可用于实时共聚焦，高分辨率或宽视场荧光显微镜以及流式细胞术。可以通过氩激光在488nm激发探针以产生对环境敏感的荧光。它的光稳定性低于罗丹明荧光探针。金畔生物是AATBioquest的中国代理商，为您提供优质的葡萄糖转运/血糖通道荧光探针2-NBDG。
NT1014,anovelbiguanide,inhibitsovariancancergrowthinvitroandinvivoAuthors:LuZhang,JianjunHan,AmandaLJackson,LeslieNClark,JoshuaKilgore,HuiGuo,NickLivingston,KennethBatchelor,YajieYin,TimothyPGilliamJournal:JournalofHematology&Oncology(2016):91
产品名称：RGD-荧光近红外750
分子量：~1600
激发波长(nm)：757
发射波长(nm)：779
近红外荧光光学成像（又称活体成像）是一种在分子水平上研究小动物疾病的技术。水和生物组织在近红外（650-900纳米）中具有小的吸光度和自荧光，从而允许有效的光子进出组织，且组织内散射较低。通过这个近红外光线，生理上丰富的分子如血红蛋白、氧血红蛋白和脱氧血红蛋白对光的吸收也降到低。具有生物相容性荧光剂的活体成像系统正在开发和优化，以用于近红外光谱区域-确保大的光穿透和高的灵敏度。细胞粘附分子整合素参与多种肿瘤的血管生成和转移。整合素在活化的内皮细胞和快速生长的实体瘤细胞中明显上调，而在静止的血管和大多数正常组织中表达低。iFluor750标记的单体精氨酸-甘氨酸-天冬氨酸（RGD）肽c（RGDyK）专门针对细胞培养和活体内的整联蛋白。iFluor750-c（RGDyK）的体内功效取决于许多因素，包括受体亲和力，特异性，分子大小，总分子电荷，亲水性和代谢稳定性。
99mTc-Glu-c(RGDyK)-BombesinSPECTCanReduceUnnecessaryBiopsyofMassesThatAreBI-RADSCategory4onUltrasonographyAuthors:JiT,GaoS,LiuZ,XingH,ZhaoG,MaQ.Journal:JNuclMed(2016):1196
InVivoBiodistributionandAnti-TumorEfficacyEvaluationofDoxorubicinandPaclitaxel-LoadedPluronicMicellesDecoratedwithc(RGDyK)PeptideAuthors:ChenY,ZhangW,HuangY,GaoF,FangX.Journal:PLoSOne(2016):e0149952
PreliminaryTherapyEvaluationof(225)Ac-DOTA-c(RGDyK)DemonstratesthatCerenkovRadiationDerivedfrom(225)AcDaughterDecayCanBeDetectedbyOpticalImagingforInVivoTumorVisualizationAuthors:PandyaDN,HantganR,BudzevichMM,KockND,MorseDL,BatistaI,MintzA,LiKC,WadasTJ.Journal:Theranostics(2016):698
c(RGDyK)-decoratedPluronicmicellesforenhanceddoxorubicinandpaclitaxeldeliverytobraingliomaAuthors:HuangY,LiuW,GaoF,FangX,ChenY.Journal:IntJNanomedicine(2016):1629
Dual-functionalc(RGDyK)-decoratedPluronicmicellesdesignedforantiangiogenesisandthetreatmentofdrug-resistanttumorAuthors:ChenY,ZhangW,HuangY,GaoF,FangX.Journal:IntJNanomedicine(2015):4863
EnhancedantitumorefficacybycyclicRGDyK-conjugatedandpaclitaxel-loadedpH-responsivepolymericmicellesAuthors:GaoY,ZhouY,ZhaoL,ZhangC,LiY,LiJ,LiX,LiuY.Journal:ActaBiomater(2015):127
ComparisonoftwonewangiogenesisPETtracers68Ga-NODAGA-E[c(RGDyK)]2and(64)Cu-NODAGA-E[c(RGDyK)]2;invivoimagingstudiesinhumanxenografttumorsAuthors:OxboelJ,Brandt-LarsenM,Schjoeth-EskesenC,MyschetzkyR,El-AliHH,MadsenJ,KjaerA.Journal:NuclMedBiol(2014):259
TheC(RgdyK)-conjugatedFe3O4nanoparticleswithhighdrugloadfordual-targetingintegrinalpha(v)beta3-expressingcancercellsAuthors:GuoL,DingW,ZhengLM.Journal:JNanosciNanotechnol(2014):4858
Designandbiologicalevaluationof(9)(9)mTc-N(2)S(2)-Tat(49-57)-c(RGDyK):ahybridradiopharmaceuticalfortumorsexpressingalpha(v)beta(3)integrinsAuthors:Ocampo-GarciaBE,Santos-CuevasCL,DeLeon-RodriguezLM,Garcia-BecerraR,Ordaz-RosadoD,Luna-GuitierrezMA,Jimenez-MancillaNP,Romero-PinaME,Ferro-FloresG.Journal:NuclMedBiol(2013):481
Quantitativepositronemissiontomographyimagingofangiogenesisinratswithforelimbischemiausing(68)Ga-NOTA-c(RGDyK)Authors:KimJH,KimYH,KimYJ,YangBY,JeongJM,YounH,LeeDS,LeeJS.Journal:Angiogenesis(2013):837
葡萄糖是一种单糖，是生物学中重要的碳水化合物。它是细胞生长的能量和代谢的中间产物。作为光合作用的主要产物之一，葡萄糖在原核生物和真核生物中都开始细胞呼吸。葡萄糖水平是许多代谢性疾病例如糖尿病的关键诊断参数。该Amplite比色法葡萄糖定量试剂盒提供了一种快速，灵敏的葡萄糖测量方法。它使用基于葡萄糖氧化酶的酶偶联反应通过产生过氧化氢来检测葡萄糖，该过氧化氢由我们的AmpliteRed过氧化物酶底物进行检测。AmpliteRed过氧化物酶底物可通过酶标仪在约570nm处读取。该测定可以轻松适用于需要测量葡萄糖的多种应用。该测定法具有非常低的背景，因为它在红色可见光范围内运行，可明显降低来自生物样品的干扰。使用Amplite比色法葡萄糖定量试剂盒，可以检测低至3μMD-葡萄糖。金畔生物是AATBioquest的中国代理商，为您提供优质的Amplite比色法葡萄糖定量试剂盒。
准备并添加葡萄糖标准品和/或测试样品（50μL）准备并添加葡萄糖分析工作溶液（50μL）在37°C孵育10-30分钟检测OD值=570nm左右的吸光度
1.储备溶液配制
除非另有说明，否则所有未使用的储备溶液应分成一次性等分试样，并在制备后储存在-20°C。避免反复冻融循环。1.1Amplite红色储备液（250X）：将100μLDMSO（组分E）加入到AmpliteRed底物（组分A）的小瓶中。应立即使用原液。任何剩余的溶液应等分并在-20℃重新冷冻。注意：避免反复冻融循环。注意：在硫醇如二硫苏糖醇（DTT）和2-巯基乙醇存在下，AmpliteRed底物不稳定。反应中DTT或2-巯基乙醇的浓度应不高于10μM。AmpliteRed底物在高pH（>8.5）下也不稳定。因此，反应应在pH7-8下进行。建议使用提供的分析缓冲液（pH7.4）。
1.2辣根过氧化物酶（HRP）储备液（10U/mL）：将1mL测定缓冲液（组分B）加入到辣根过氧化物酶（组分C）的小瓶中。注意：未使用的HRP溶液应分为单次使用的等分试样并储存在-20℃。
1.3葡萄糖氧化酶溶液（100U/mL）：将1mL测定缓冲液（组分B）加入葡萄糖氧化酶（组分D）的小瓶中。注意：未使用的葡萄糖氧化酶溶液应分为一次性等分试样并储存在-20℃。
1.4葡萄糖原液（800mM）：将1mL测定缓冲液（组分B）加入葡萄糖小瓶（组分F）中。注意：未使用的葡萄糖溶液应分为单次使用的等分试样并储存在-20℃。
2.标准溶液配制
2.1葡萄糖标准溶液配制通过将适量的800mM葡萄糖储备溶液稀释到测定缓冲液（组分B）中来制备葡萄糖标准品，以产生30μM的葡萄糖浓度。然后在测定缓冲液（组分B）中进行1：3连续稀释，得到约10,3,1,0.3,0.1和0.03μM连续稀释的葡萄糖标准品。包括非葡萄糖缓冲液对照作为空白对照。
3.工作溶液配制
表1.一个透明底96孔微孔板的分析工作溶液（2X）
表2.固体黑色96孔微孔板中葡萄糖标准品和测试样品的布局。
GS=葡萄糖标准品（GS1-GS7）;BL=空白对照;TS=测试样品。
表3.每个孔的试剂组成
注意：由于Amplite红色底物（对非荧光产物）的过氧化，高浓度的葡萄糖（例如测试样品或标准品中的100μM）可能导致荧光信号减少。
葡萄糖测定
1.如表2和3中所述，将葡萄糖标准品和含葡萄糖的测试样品加入96孔黑色微孔板中。
2.将50μL葡萄糖测定工作溶液添加到葡萄糖标准品，空白对照和测试样品的每个孔中（表2），以使总葡萄糖测定体积为100μL/孔。注意：对于384孔板，每孔加入25μL样品和25μL测定反应混合物。
3.将反应在37℃孵育10至30分钟，避光。
4.用荧光板读数器在Ex/Em=530-570nm/590-600nm（Ex/Em=540/590nm）监测荧光强度。
数据分析
从空白标准孔获得的读数（RFU）用作阴性对照。从其他标准的读数中减去该值，以获得基线校正值。然后，绘制标准读数以获得标准曲线和方程。该等式可用于计算葡萄糖样品。点击使用在线线性回归计算器。
图1.使用Gemini酶标仪（MolecularDevices），在96孔黑色平板上用Amplite荧光葡萄糖定量试剂盒测量葡萄糖剂量反应。
试剂应用文献
准备并添加葡萄糖标准品和/或测试样品（50μL）准备并添加葡萄糖分析工作溶液（50μL）在37°C孵育10-30分钟在Ex/Em=540/590nm处检测荧光强度
上海金畔生物科技有限公司代理日本Sibata柴田科学环境仪器全线产品，欢迎访问日本Sibata柴田科学环境仪器官网了解更多信息。
A65650-001A65650-002A65650-004A65650-005A65650-006A65650-007D95000-111D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-005A65650-006A65650-007D95000-111D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-006A65650-007D95000-111D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-007D95000-111D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-006D95000-111D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-006A65650-007D95000-112D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-006A65650-007D95000-111D95000-113D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-006A65650-007D95000-111D95000-112D95000-114D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
A65650-001A65650-002A65650-003A65650-004A65650-005A65650-006A65650-007D95000-111D95000-112D95000-113D95000-115A65130-001A65650-008A65650-009A65650-010A65650-011
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