小猪可能会解锁人类体外受精的钥匙

R.MichaelRoberts,MU策展人,动物科学杰出教授,邦德生命科学中心研究员,RandallPrather,农业,食品和自然资源学院动物科学着名教授,与猪一起研究干细胞和这些细胞用于在体内增殖,交流和生长的机制。在试图改善这些细胞生长方式的过程中,他们实验室的研究人员发现了一种使用特殊液体培养基的方法,可以提高猪体内试管的成功率。

“这真是一个偶然的发现,”罗伯茨说。“一般来说,生产有活力的胚胎有多个步骤,然后我们可以植入参与我们研究的猪和奶牛;然而,这是昂贵的,有时收益很少。我们正在寻求一种方法来更有效地做到这一点,并偶然发现了一种可能对人类生育诊所产生影响的方法。“

在涉及猪的体外受精(IVF)中,科学家首先从雌性猪中提取卵母细胞或卵,以及围绕它们的“护士”细胞,并将它们置于旨在使卵成熟的化学环境中。然后使卵子受精以产生受精卵,或允许发育六天的单细胞胚胎。然后将这些胚胎转移回雌性猪,希望成功怀孕并健康仔猪。

罗伯茨实验室前研究助理教授叶元和动物科学高级研究专家李斯帕特的任务是在植入前提高仔猪胚胎的效率和质量。

在一项研究中,研究小组分析了培养猪干细胞时使用的各种特殊生长因子,并添加了两种因子-成纤维细胞生长因子2(FGF2)和白血病抑制因子(LIF)。他们发现,当加入第三种因子-胰岛素样生长因子时,这种组合产生了特殊的液体环境,卵母细胞需要能够进行受精和进一步发育成能够成功怀孕的胚胎。

这三种化合物共同创造了一种名为“FLI”的化学介质,可以彻底改变仔猪和人体IVF治疗;已通过MU技术管理和工业关系办公室提交了专利申请,以鼓励新方法的商业化。

“它改善了整个过程的各个方面,几乎使卵母细胞成熟的效率提高了一倍,”罗伯茨说。“无论何时你做科学,你都会认为你正在做一些有用的事情。当我们开始时,它不是为了改善女性的生育能力,而是为了让猪得到更好的卵母细胞。现在,它可能是FLI中可能会成为牛胚胎工作的重要,甚至可能与人IVF帮助。”

THE END
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